Microbe protects honeybees by boosting nutrition

Indiana University researchers have identified a specific bacterial microbe that, when fed to honeybee larvae, can reduce the effects of nutritional stress on developing bees — one of the leading causes of honeybee decline.

Humans rely on honeybees for food security. Because they will pollinate almost anything, honeybees are extremely useful for agriculture. But over the past few decades, the honeybee population has experienced dramatic declines caused by multiple stressors, the most pervasive of which is limited nutrition. 

U.S. beekeepers reported losing 40.5 per cent of their managed colonies between 2015 and 2016 alone, according to a national survey.

“The effects of poor nutrition are most damaging in the developing larvae of honeybees, who mature into workers unable to meet the needs of their colony,” said Irene Newton, a professor in the IU Bloomington College of Arts and Sciences’ Department of Biology, who led the study. 

Newton said honeybees need to collect pollen and nectar from a variety of plants and flowers to help colonies stay healthy. But many bees in the U.S. lack floral diversity.

“We’ve changed the way we use our land in the U.S.,” Newton said. “Now we have tons of monoculture crops like corn, which are wind pollinated and therefore no use to bees, covering acres and acres of land. Other crops that bees do pollinate are grown in monoculture as well, limiting the options for bees.

“If you limit yourself to only eating one thing, that’s not healthy for you. You have to have a broad diet that will help fulfil all of your nutritional needs. Bees are the same way.”

Honeybee larvae are fed by their sister bees. Their diet consists of foraged ingredients such as nectar and pollen, as well as royal jelly — a bee glandular secretion that is complex and nutrient rich. If larvae are destined to be queens, they will eat royal jelly their whole lives. If they are workers, their diets will shift to nectar and pollen after a few days.

In addition to being more nutritious than nectar and pollen, royal jelly has long been known to possess potent antimicrobial properties due to its acidity, viscosity and the presence of antimicrobial peptides. This means that most microbes exposed to royal jelly die, Newton said.

Except one.

According to their new study, Newton and her research team found that a specific microbe — Bombella apis — is the only larva-associated bacterium that’s able to thrive in royal jelly. They also found that B. apis makes royal jelly more nutritious by significantly increasing its amino acid content, which helps developing bees build resilience against nutritional stress.

“We have identified a nutritional symbiont of honeybees — a microbe that can help bolster the bees against nutrient scarcity and stress,” Newton said. 

“When we limited bee nutrition during development, we saw a drop in mass for the bees; bees were much smaller than their control counterparts.

“When B. apis was added to these same bees, although they had poor nutrition, they reached the same mass as control bees given full nutrition. The microbe was able to make up for the poor diet. This suggests that B. apis could be added to colonies as a probiotic to protect from nutritional stress.”

B. apis may have potential as a key supplement in future beekeepers’ efforts to counteract the negative influence of poor nutrition on honeybee health. B. apis can survive for more than 24 hours in sugar water, so beekeepers who are already supplementing colonies could potentially integrate a B. apis probiotic into their bees’ diets.

This research expands on more than six years of previous studies by Newton and her colleagues, including findings that B. apis protects bees against fungal infections and is a significant part of the queen gut microbiome.

“We are excited to explore the other interactions that B. apis has in a colony, to better understand what it’s doing in different environments and the role it plays in association with honeybee queens,” Newton said.